[Alterations of immunebalance in the rats blood during experimental preeclampsia].

The aim of the study was to establish a violation of hormonal (progesterone and estradiol) and immune balance (interleukins IL-2, IL-10, TNF-α) in the rats blood at different stages of experimental PE induced by hypoxia placenta. For the purpose of modeling of PE in pregnant rats, at 10-thday of gestation the lumen of the abdominal aorta below the renal artery was narrowed by the silk thread a third of its diameter (0.2 mm). Blood for the study was taken at the18thand25th day of pregnancy. In the blood content of interleukins(IL-2, IL-10, TNF-α) and the concentration of progesterone and estradiol by immunoenzymaticassay were measured. A results of research established that with increasing of the gestational age in the rats with experimental PE estradiol and progesterone content in the blood increased much slower than in the control animals. At the same time in the blood of pregnant rats with placental hypoxia in the second trimester TNF-α level was by 80% higher and the level of IL-10 was 30% lower than in the control animals. With the increase of gestational age (in the third trimester) in animals of the experimental group blood content of IL-2 and TNF-α increased sharply (by 50% and 30%), whereas the level of IL-10 did not change compared with baseline values. In the control group of animals, alterations in the cytokines content were much slower. It was concluded that violation of the synthesis of progesterone in hypoxic placenta and its insufficient content in the blood during pregnancy is one of the causes of disorders of the immune balance in the body of pregnant rats. Observation of the dynamics of progesterone in the blood during pregnancy may be informative for the early diagnosis of PE and its timely treatment.

[Morphological changes in brain and heart after the temporary liver exclusion from the bloodstream during the cholestasis].

The aim of the study was the detection of the peculiarities of the changes in brain and heart, developed by the cholestasis after ischemia (due to the temporary liver exclusion from the bloodstream) and post-ischemic restoration of bloodstream. The experimental study was carried out on 12 mature rats (weight 150-200 g). After the creation of 5 day cholestasis pattern, the exploration and clamping of the hepato-duodenal ligament was done for 10 minutes. After the 15 minutes, 24 hours and 48 hours of the clamp withdrawal from the portal complex, the pieces of the heart and the brain were investigated. The aether narcosis was applied for the anaesthesia purpose. The changes peculiar to the brain damage (perivascular and pericellular swelling, proliferation of glia and neurophagia) were observed shortly after the removal of the ischemia. The mentioned changes were dramatic after 24-48 hours of experimentation. The study suggests that the dramatic changes were developed in the brain and cardiac tissues after 15 min. of the experimentation. Mentioned changes are dismetabolic processes and they continuing in the later terms, including the 48 hours later of the experimentation.

[Common intralobular microcirculatory module peculliarities in cholestasis in white rats].

The dynamics of intralobular microcirculatory module transformation in cholestasis was investigated. The liver tissues of 54 white Wistar rats were studied by Histology, TEM, TEM after injection of 1% Pb(NO(3))(3), vie common bile duct (CBD), SEM of corrosion casts prepared after methylmethacrylate injection vie CBD. It is demonstrated that liver is the basis of microcirculation of 4 different liquids: blood, bile, connective tissue liquid and lymph. Accordingly, in normal condition 4 different well formed compartments bordered from each-others by different types of cells exist. In the yearly stages of cholestasis (3-6 days) the function of bile drainage is partially overtaken by lymph pathways. This is accompanied by the beginning of the destruction of structures bordering above-mentioned microcirculatory beds. In the later stages of cholestasis (12-18 days) the final disorganization of microcirculatory compartments with mixture of all liquids is observed. It is accompanied by increased dystrophy of liver cells population.